Abstract

EFFECTS OF ETHANOL LEAF EXTRACT OF TAPINANTHUS BANGWENSIS ON HEPATIC REDOX AND INFLAMMATORY PROFILE ON CARRAGEENAN-INDUCED PAW OEDEMA IN WISTAR RATS

O. O. Babalola, O. T. Adedosu*, J. A. Badmus, G. E. Adeleke and E. O. Olagoke

ABSTRACT

Studies have shown links between oxidative stress and pathogenesis of inflammatory diseases. This study investigated the effect of ethanol leaf extract of Tapinanthus bangwensis on hepatic redox and inflammatory profiles in carrageenan-induced paw oedema in Wistar rats. Sixty male Wistar rats weighing averagely 180g, were selected randomly into 8 groups and treated: Group I (Control, distilled water for 21 days), Group II (orally received 200 mg/kg body weight (b.w) of extract repeatedly for 21days), Group III (received 10 mg/kg b.w of Naproxen for 21 days), Group IV (received 1% carrageenan intraperitonially for 5 hours), Groups V, VI, VII (were pre-treated orally with 100, 200, and 400 mg/kg b w respectively of extract repeatedly for 21days and treated with 1% carrageenan intraperitonially), Group VIII (received 10 mg/kg body weight of Naproxen for 21 days and treated with 1% carrageenan intraperitonially to induce oedema at the hind paw of the rats). After 5 hours, animals were sacrificed and the oedema paws were cut for further analysis. Biochemical indices studied includes, Total Protein (TP), White Blood Cell (WBC) counts, Platelet count, Nitric oxide (NO) concentration, Aspartate amino transferase (AST), Alanine Amino Transferase (ALT), Alkaline Phosphatase (ALP), Myeloperoxidase (MPO) activities, Reduced Glutathione (GSH), Malondialdehyde (MDA) concentration, Superoxide Dismutase (SOD) and Catalase (CAT) activities all determined by standard methods. Inflammatory markers such as WBC counts, Platelet count, NO concentration and MPO activity were significantly (p<0.05) increased in the carrageenan treated group but were reversed in the pre-treated groups (V,VI,VII) in a dose dependent manner, while 200 mg/kg dose of extract was effective comparably with standard drug. Pre-treatment group with the extract showed improved liver function even with the carrageenan treatments. The extract also showed improved liver protein synthesis as well as MDA reducing capacity even with the induction of oedema using carrageenan administration in both pre-treatment and post-treatment group comparably with treatment using the standard drug. Ethanol leaf extract of Tapinanthus bangwensis ameliorated carrageenan-induced hepatic damage and paw oedema in the rats via correction of hepartic redox imbalance, antioxidative and anti-inflammatory activities. The bioactive component of the extract may serve as template for drug design in management of diseases related with oxidative stress and discovery of novel anti- inflammatory agent against oedema.

Keywords: Tapinanthus bangwensis, Carrageenan, Redox imbalance, Antioxidative, Anti-inflammatory, Paw oedema.