Abstract

PANAX NOTOGINSENG EXTRACTS: ANTICOAGULATION, DNA PROTECTION EFFECTS

Tri Le Nguyen Ngo and Tu Nguyen*

ABSTRACT

Panax notoginseng was first formally reported in Li Shi Zhen's "Materia Medica," a Chinese herb dictionary (AD 1518-1593). Modern pharmacological research on Panax notoginseng has discovered that it has a variety of effects on the blood, cardiocerebal vascular system, central nervous system, endocrine system, inflammation, and so on. To study the biological activities and potencies through phytochemical tests of anticoagulation activities, DNA protection effects and phytochemicals from Panax Notoginseng extracts in Vietnam. Panax Notoginseng was soaked in 70% methanol and then separated into two components: methanol suspension and residue. The methanolic suspensions were evaporated to collect the methanolic cataplasm, then mix with either distilled water or PBS. The first part of residue was continuously boiled for six hours at 85oC to collect the boiled root cataplasm, combine B cataplasm with PBS and the second one wasreconstituted and ground with either methanol or distilled water. After that, a 5g of residue grounded with methanol was collected and kept at -20oC to generate oil extract. In vitro anticoagulation activity, clotting test and thrombolytic test were conducted to determine the effect of different extracts obtained from Panax Notoginseng. In addition, the spot assay was used to evaluate the DNA damage protection of the methanolic cataplasm mix with distilled water (10 mg/mL, 100 mg/mL) on Saccharomyces cerevisiae ATCC 9763 under the effect of hydrogen peroxide and hydroxyurea. In this study, Panax Notoginseng showed strong anticoagulant and thrombolytic potential at almost all samples. Panax Notoginseng also shown the ability to reduce the impact of hydrogen peroxide at low concentration (10 mg/mL). However, its capacity to prevent the harm caused by hydroxyurea remained inconclusive. TLC results indicate that the aqueous root sample extract may include Apigenin and Ginsenoside Rb1, while the methanolic suspension sample may have only Apigenin. The biological activities of Panax notoginseng had been well understood with the findings of both in vitro studies, increasing the potential of developing the extracts for use in drug discovery. For deeper analysis and more precise data, purification processes must be performed.

Keywords: Panax Notoginseng, anticoagulation activities, extracts, DNA damage protection.