Abstract

EVALUATION OF THE ANTIBIOTIC RESISTANT PLASMID TRANSFERING ABILITY OF SALMONELLA ENTEROTICA S17 INTO ESCHERICHIA COLI

Nguyen Ngo, Vu Truong and Tu Nguyen*

ABSTRACT

One of the most commonly isolated foodborne pathogens is Salmonella. More than 2500 Salmonella serotypes have been recognized so far, and more than half of them are associated with Salmonella enterica subsp. enterica. The development of multi – drug resistant (MDR) Salmonella serotypes is also having a substantial influence on the effectiveness of antibiotic therapy, and an increase in the prevalence of MDR strains may enhance the mortality rates associated with Salmonella infections. This study would transfer the antibiotic resistance genes from S. kentucky 1067 into E. coli JM109 and evaluate the multi – drug resistant rate of transformed plasmid. Plasmids carrying resistant genes were isolated from S. kentucky 1067 using alkaline lysis method and were transformed into E. coli JM109. For the antibiotic susceptibility test, disk diffusion method was used for the determination of the multi – drug resistant phenotype of S.kentucky 1067 via transformed E. coli with two different antibiotics. S. kentucky 1067 was recorded to resist both tested antibiotics including Ampicillin (AM) and Ciprofloxacin (CIP). Besides, 100% transformed E. coli clones were shown their resistance to AM. The proportion of E. coli containing plasmids extracted from Salmonella that resisted to at least two antibiotics was 83.33%, for CIP. Therefore, understand thoroughly the characteristics of these plasmids would make a significant contribution in the future.

Keywords: Antibiotic resistance, Salmonella kentucky 1067, Plasmid, transformation.