Abstract

INFLUENCE OF RATIO OF WATER TO MATERIAL ON POLYPHENOL AND SCREENING ANTI-BACTERIA ACTIVITY OF CONDONOPSIS PILOSULA ROOTS

Anh Le, Tram Pham and Tu Nguyen*

ABSTRACT

Codonopsis pilosula (C. pilosula) is a traditional herbal medicine that has been commonly used as a nutrional and medicinal supplement in several oriental countries for a hundred years. In this project, the C. pilosula was harvested from Komtum (Vietnam) and went through an optimization polyphenol extract using ultrasound-assisted extraction (UAE) to check the effect of ratio of solvent to material. The Follin-Cioallute assay was used to find the total phenolic concentration (TPCs). The optimal extraction conditions were ethanol at 69.49oC, a solvent to material ratio of 39.49, and an extraction period of 117.88 minutes (about 2 hours). Under these conditions, the optimum concentrations of the polyphenol were 8.01 mg gallic acid equivalent (GAE)/g dry weight (DW). In addition, screening the bioactivates using agar well diffusion to estimate the tyrosinase and bacterial from C. pilosula (500 mg/mL) had remarkably potent anti-bacterial properties when compared to absolute ethanol, despite variations in the zones of inhibition brought on by the various test microorganisms. In essence, the extraction of polyphenols from C. pilosula using UAE is regarded as a natural source of anti-bacterial activity. To fully comprehend its therapeutic benefit, it was crucial to carry out additional research using active substances isolated from C. pilosula root and to optimize different extraction methods.

Keywords: Codonopsis pilosula, Ultrasound-assisted extraction (UAE), Polyphenol, phenolic compounds, Anti-bacterial activity.