Abstract

METHOD DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD OF LORNOXICAM IN BULK DRUG AND IT`S DOSAGE FORM

Akshay Shimpi*, Rajesh Jadhao, Fariyal Shaikh, Ajay Rathod and Akshay Patil

ABSTRACT

An HPLC-PDA method was developed and validated for the determination of hydrochlorothiazide in bulk and pharmaceutical formulation. The method was optimized selecting chromatographic conditions of Methanol: water (0.05%OPA), (60:40) v/v, pH 3.was used as the mobile phase for the method. The detection wavelength was 307 nm and flow rate were 0.7ml/min. In the developed method, the retention time of Lornoxicam were found to be 3.699 min. The developed method was validated according to the ICH guidelines. The linearity, precision, range, robustness was within the limits as specified by the ICH guidelines. So the proposed methods can be used for the routine quality control analysis Lornoxicam in bulk drug as well as in formulations.

Keywords: Method development and validation, Lornoxicam, Tablets, RP- HPLC.