Abstract

A NOVEL UV-SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF CIPROFLOXACIN HYDROCHLORIDE IN BULK AND PHARMACEUTICAL DOSAGE FORM

Suresha D. N.* and Somashekhar C. N.

ABSTRACT

A novel, simple, accurate and precise Area under curve spectroscopic method was developed and validated for the estimation of Ciprofloxacin Hydrochloride in bulk and Pharmaceutical dosage forms and has an absorption maximum between 267-277 nm in distilled water. The Linearity was found to be in the concentration range of 2- 12 μg/ml and the correlation coefficient was found to be 0.999 and it has showed good linearity, reproducibility, precision in this concentration range. The regression equation was found to be Y = 0.083X+ 0.002. The % recovery values were found to be within 100.02 -100.43 % showed that the method was accurate. The LOD and LOQ were found to be 0.0562 and 0.562 μg/ml, respectively. The % RSD values were less than 2. The method has been validated according to ICH guidelines for linearity, accuracy, precision, robustness, ruggedness. Limit of detection and limit of quantitation. Proposed method was successfully applied for the quantitative estimation of Ciprofloxacin Hydrochloride in bulk and pharmaceutical dosage form.

Keywords: Ciprofloxacin HCl, AUC UV- Spectroscopy, Distilled water, Accuracy.