Abstract

EVALUATION OF ANTI DIABETIC ACTIVITY OF BLACK RICE

S. Meera, Anandhalakshmi J. and Dr. V. Hemamalini*

ABSTRACT

Consumption of junk foods, new varieties of white rice shows more probability of getting affected with health problems such as diabetes, cholesterol related health effects and deficiencies. In order to regain the good health, proper food should be taken in. This includes intake of healthy food such as naturally grown food crops, traditional foods. Less popular than red or brown rice, black rice is eaten commonly in many parts of Asia and since ancient times. It is also known as the forbidden rice and has impressive health benefits. This present study is done in order to compare and re-evaluate the extracts of a well- known Indian Rice variety Black rice (Karunkuruvai) with White rice (Ponni) for their anti-diabetic property for achieving precision and perfection. The phytochemical screening of Black (karunkuruvai) and white (ponni) rice varieties, over solvents such as ethanol, methanol, petroleum ether, acetone and distilled water reveals the presence of flavonoids and tannins in Black rice (karunkuruvai). Quantitative estimation of total carbohydrate present in black and white rice was done using phenol sulfuric acid method where black rice contains lower amount of carbohydrates compared to that of white rice in its highest concentration. Black rice possessed 11.25(μg/ml) and white rice possessed 14.51(μg/ml). Estimation of Anthocyanin was carried out where black rice contained 19.34mg of anthocyanin in 1 gram of powdered sample and white rice with 11.81mg of anthocyanin in 1 gram of powdered sample. Aqueous extract of black rice was tested for alpha amylase and alpha glucosidase where the highest activity was shown in 100μg concentration and lowest concentration shows the lowest effect. While, 60 μg/ml concentration have showed the 50% inhibition(IC50). RIN 5F cell line work has been done for cell viability where 50 μg/ml concentrations have showed the 50% inhibition (IC50) of diabetes. Hence it is concluded that the black rice has the ability to maintain the blood glucose level and thereby has anti-diabetic activity.

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