Abstract

ISOLATION AND CHARACTERIZATION OF BACTERIA FROM FRESH FRUIT JUICES AND THEIR ANTIBIOTIC PROFILE

*Aarti, Nisha Dhillon, Shalu Choudhary and Ayush Tomar

ABSTRACT

Aim: Due to the lack of knowledge regarding the relationship between the vendor's sanitary status and the level of contamination, a descriptiv study was conducted in order to add to the body of comparable studies and assist the government in developing the best possible policy. Fruit juices are widely consumed in many tropical nations and are known fo their high nutritional value, mineral content, and vitamin content. Methodology: The most probable number [MPN] test was used to quantify the coliform population in both water and juice samples after a sample of various fruit juices was gathered from five different public hotspot places in Meerut. The research was then expanded to include the isolation of coliforms using a selective solid media and the detection of several Enterobacteria species utilising quick biochemical tests. Five fruit juice samples were gathered from five different vendors, grown on [NAM, MSA, EMB, MCA] agar, subjected to pertinent biochemical analyses, and isolated. To get the average CFU for the vendor, the CFU of isolates purchased from one vendor were averaged. The sellers were questioned, and the investigator also recorded his observations of the vendors. Both were used to create an inventive hygiene score [VHS] that was utilised to determine the vendor hygiene score. The scatter plot and spearman's rho were computed after statistical analysis of the vendors' average CFU and VHS. Result: Based on the MPN studies, juice sample-1 was found to be most contaminated with a count of 3 microbes/50ml, sample -2 with a count of 2 microbes/ 50ml, sample-3 with a count of 3 microbes/50ml, sample-4 was 4 microbes/50ml.The least count of only 2 microbes /50ml was observed in sample-5. The colony characteristics, microscopic examination as well as rapid biochemical studies showed the presence of different pathogenic bacteria all five fresh fruit juices samples. Bacillus, Staphylococcus aureus, Lacto-bacillus, Streptococcus, Streptobacillus. Bacillus and Staphylococcus aureus are the major bacterial and contaminant. The median VHS is -9, which is unsuitable for consumption according to HSS. Spearman's rho was -0.736, suggestive of a statistically significant negative nonlinear correlation between VHS and extent of microbial contamination in street-vended fruit juices. Conclusion: To improve public health, lower disease mortality and morbidity, reduce economic losses, and protect the general public from the risk of bioterrorism through strict surveillance against the backdrop of limited employment opportunities and constrained livelihoods of the vendors, the abundance of microbial contamination of street-vended fruit juices is a lucrative dimension for fruit juice microbiology research. The first three fruit drinks also had substantial amounts of contamination, according to the MPN study. This is conceivable because the water used to make juice is of poor quality. Water is also a significant cause of sewage contamination.

Keywords: Fresh Fruits juice, Gram staining, Antibiotic, Bacillus, staphylococcus aureus.