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SIMULTANEOUS ESTIMATION OF METFORMIN AND EMPAGLIFOZIN BY USING RP-HPLC METHOD IN ITS BULK AND PHARMACEUTICAL DOSAGE FORM
Boddu Revathi*, Laxmi Purnima Yadav, Sushma Daram, Sarika Bommena
ABSTRACT A simple, Accurate, precise method was developed for the simultaneous estimation of the Metformin and Empagliflozin in Tablet dosage form. Chromatogram was run through Std Symmetry 150 x 4.6 mm, 5. Mobile phase containing 0.1% OPA Buffer: Acetonitrile taken in the ratio 60:40 was pumped through column at a flow rate of 1ml/min. Buffer used in this method was 0.1% OPA buffer. Temperature was maintained at 30°C. Optimized wavelength selected was 230 nm. Retention time of Metformin and Empagliflozin were found to be 2.219min and 2.794min. %RSD of the Metformin and Empagliflozin were and found to be 0.6 and 0.7 respectively. %Recovery was obtained as 100.76% and 99.90% for Metformin and Empagliflozin respectively. LOD, LOQ values obtained from regression equations of Metformin and Empagliflozin were 0.07, 0.22 and 0.088, 0.025 respectively. Regression equation of Metformin is y =27484x + 19205, and y =21756x + 408.7 of Empagliflozin. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries. Keywords: Metformin, Empagliflozin, Simultaneous estimation, Method Development, RP-HPLC. [Download Article] [Download Certifiate] |