Abstract

DEVELOPMENT AND VALIDATION OF ANALYTICAL METHOD FOR THE ESTIMATION OF CEFTAZIDIME IN THE PHARMACEUTICAL DOSAGE FORM

Devi Swapna P.V.*, Fathima A., Lakshmi Mohan, Muhsina Salim, Nikhil K. V. and Sreeshma M.

ABSTRACT

A UV/Vis spectrophotometric and RP-HPLC method has developed and validated for the estimation of ceftazidime in pharmaceutical dosage form. UV spectrophotometric method utilises Systronics double beam UV spectrophotometer using 1.0 cm quartz cells and all determinations were made at wavelength of 256.2 nm in acetonitrile. The RP-HPLC utilizes analytical high performance liquid chromatography Analytical Tech using the C18 (octadecyl) column using mobile phase water and acetonitrile in the ratio 70:30 at a flow rate of 1.0 ml/min. detection were carried out at UV detector at 256.2 nm and obtains the retention time at 2.34 min. both the methods were validated according to ICH guidelines. The proposed method was found to be linear in the concentration ranges from 2-16 μg/ml with the linear correlation coefficient of R2 = 0.998 and the mean recoveries were 99.88 to 99.95% in UV spectrophotometric studies. The regression coefficient was equal to 0.998 and mean recovery was found to be 99.86 to 99.95% in RP-HPLC studies. The detection limits and quantification limits were found to be 0.110 and 0.333 μg/ml for the spectroscopic method and 0.093 and 0.283 μg/ml for the RP-HPLC method.

Keywords: UV-Vis spectrophotometer; Method validation; RP-HPLC method; Recovery studies.