Abstract

ACUTE AND SUB CHRONIC TOXICITY OF ETHANOLIC EXTRACT OF SYZYGIUM CUMINI LINN LEAVES

Ch. Guru Anitha, *C. Bhuvaneswara Rao and Dr. P.V. Madhava Reddy

ABSTRACT

Most of these investigations are performed to determine the toxicity of substances to humans, animals, or the environment; to learn more about the mechanism of hazardous chemicals; or to provide better methods for detecting certain chemically caused effects. In light of OECD recommendations, the current study set out to assess the acute and 28-day repeated oral toxicity of ethanolic extract of Syzygium cumini Linn leaves. Animals in an acute oral toxicity study were given a dose of 2000 mg/kg of the plant extract and then monitored every 30 minutes for the next 1, 2, and 4 hours. Four groups of six rats each were used to conduct a repeated dose, 28-day oral toxicity investigation. The animals in Group 1 were used as a comparison. Animals in Group II were given a low dose of plant extract (50 mg/kg), whereas those in Group III were given a moderate dose (100 mg/kg), and those in Group IV were given a high dose (200 mg/kg) (orally, once daily, for 28 days). Results from both the acute toxicity trial of plant extract at a dose of 2000mg/kg and the recurrent oral toxicity study over 28 days showed no harm or mortality. There were no discernible alterations in haematological and biochemical markers, relative organ weight, gross necropsy, or histological examination throughout a repeated oral toxicity studies of the herbal mixture over the course of 28 days. The current study's findings reveal that the newly developed plant extract is entirely safe and non-toxic for therapy, with an LD50 of >2000 mg/kg.

Keywords: Plant extract, Acute toxicity, Sub Acute toxicity, WBC, Organ Weight