Abstract

IN VITRO STUDY OF ANTIFUNGAL ACTIVITY OF PADMAK AGAD AGAINST DERMATOPHYTE

Shilpa Gawande Kolarkar* and Tushar Khairnar

ABSTRACT

Background Lesions of Dadru or Tinea are also similar to spider bite lesions hence Padmak agad (Antitoxic formulation); mentioned in the treatment of the same used here in study. Aims and objectives to find an alternative therapy which will reduce rate of relapse and recurrence of fungal infection. Methods Contents of Padmak agad were mixed with water and soaked overnight, filtrate sterilized and used for the study. Zone of inhibition of growth of the fungus was observed and compared with standard. Results Zone of inhibition formed due to Padmak agad was 11mm whereas due to control drug was 30mm.Observations though the contents formulation did not dissolve completely; the filtrate shows antifungal activity. Discussion Filtrate showed minimal antifungal activity in comparison with the control Fluconazole 1. As the coarse powder soaked more water and did not dissolve completely hence, the concentration achieved was dilute in comparison with the standard. 2. Test drug can show more inhibition zone if extracts of it is used. 3. Trichophyton mentagrophyte is the most resistant fungus species. 4. Due to filtration process many active constituents may have not participated in the activity study. 5. Sterilization might have hampered thermolabile chemical constituents showing minimal antifungal activity of Padmak agad.

Keywords: Padmak Agad, Dermatophyte, Antifungal, in-vitro, Fluconazole, Tinea.