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ISOLATION AND PARTIAL PURIFICATION OF ANTIBACTERIAL ACTIVITY PROTEIN FROM INDIAN HONEY Apis cerana indica
Vennilla S., Kathireswari P*., Karthikeyan P , and Manimegalai M
ABSTRACT
The present work has been carried out to identify the antimicrobial compound in Indian honey and the range of organisms susceptible to the antimicrobial action. Antibacterial potential of the precipitated protein, from honey was used to elucidate the antimicrobial activity. In order to purify the sample the protein was subjected to 10kDa molecular weight cut off centrifugation and the positive phase was taken for further purification by thin layer chromatography. The maximum inhibition as produced was observed against E.coli (16 to 23 mm), Klebsiella pneumonia (14 to 19 mm), Pseudomonas sp. (15 to 21 Keywords: Honey, Apis cerana indica, antibacterial protein, zone of inhibition. [Download Article] [Download Certifiate] |