Abstract

ISOLATION AND PARTIAL PURIFICATION OF ANTIBACTERIAL ACTIVITY PROTEIN FROM INDIAN HONEY Apis cerana indica

Vennilla S., Kathireswari P*., Karthikeyan P , and Manimegalai M

ABSTRACT

The present work has been carried out to identify the antimicrobial compound in Indian honey and the range of organisms susceptible to the antimicrobial action. Antibacterial potential of the precipitated protein, from honey was used to elucidate the antimicrobial activity. In order to purify the sample the protein was subjected to 10kDa molecular weight cut off centrifugation and the positive phase was taken for further purification by thin layer chromatography. The maximum inhibition as produced was observed against E.coli (16 to 23 mm), Klebsiella pneumonia (14 to 19 mm), Pseudomonas sp. (15 to 21mm) and Proteus sp. (15 to 16 mm). The present findings revealed that local Indian honey of Apis cerana indica had higher antibacterial activity against the bacterial strains. Active fraction of the gel filtration elutes showing higher concentration of proteins with Rf value 1.6 and autobiography assay conforming the zone of inhibition of TLC separated protein.

Keywords: Honey, Apis cerana indica, antibacterial protein, zone of inhibition.