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RAPID DETERMINATION OF IBUPROFEN CONCENTRATION IN HUMAN PLASMA BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Syed N. Alvi, Ahmed Yusuf, Eman Al Gaai and Muhammad M. Hammami*
ABSTRACT
A simple, precise, and rapid reversed-phase high performance liquid chromatography (HPLC) method for the determination of ibuprofen (IBU) in human plasma using gemfibrozil as an internal standard (IS) was developed and validated. Plasma samples containing ibuprofen were spiked with the IS, precipitated with methanol, and centrifuged. The compounds of interest were efficiently separated on Symmetry Shield RP18 column maintained at a temperature of 40 ºC, and detected with multi-wave length fluorescence detector (excitation/emission wavelength 230/ 275 nm). The mobile phase consisted of 0.01M dibasic ammonium phosphate (pH adjusted to 5.5 with phosphoric acid) and acetonitrile (45:55, v:v), and was delivered at a flow rate of 1.5 ml/min. The relationship between ibuprofen Keywords: Ibuprofen, Gemfibrozil, Human plasma, HPLC. [Download Article] [Download Certifiate] |