DEVELOPMENT AND VALIDATION OF UV-SPECTROSCOPIC METHOD FOR THE ESTIMATION OF DIDANOSINE IN TABLET
Mohammad Zameeruddin, Kalyankar Swapnil S*., Solanke S. B., Jadhav S. B.,Kadam V.S. and Bharkad V. B.
ABSTRACT
A simple, sensitive, rapid and accurate UV-Spectroscopic method has
been developed for the estimation of Didanosine in bulk drug and
tablet dosage form. In order to increase value of absorbtivity and
subsequently LOD the drug was dissolved in water and acetonitrile
(90:10) and absorbance was measured at 249.60 nm. The linearity of
method was found to be between 1-14 μg/ml. The method was
validated based on ICH guidelines. Hence useful for the routine
analysis of Didanosine.
Keywords: Didanosine, Acetonitrile, UV-Spectrophotometric Method, ICH Guidelines.
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