Abstract

DEVELOPMENT AND VALIDATION OF ANALYTICAL METHODS FOR SIMULTANEOUS ESTIMATION OF TELMISARTAN AND CAPTOPRIL IN SYNTHETIC MIXTURE

Jagruti Solanki*, Prof. Mitali Dalwadi, Dr. Umesh Upadhyay

ABSTRACT

Two different methods namely zero order derivative spectroscopy, RP-HPLC method have been developed and validated for estimation of Telmisartan and Captopril in synthetic mixture. Methanol was used as solvent. Zero order spectroscopic method we measured was developed in which absorbance of Telmisartan and Captopril were found at 240 nm and 262 nm respectively and show linearity (r2>0.999) over the range of 8 – 40 μg/mL and 5 – 25 μg/mL for TEL and CAP respectively. A simple, sensitive and accurate RP-HPLC method was developed for simultaneous estimation of telmisartan and captopril in synthetic mixture. The Reversed phase High Performance Liquid Chromatography Method was developed and validated for the determination of both the drugs. RP-HPLC method gives us good resolution and better separation for both the drugs. The separation was carried out by using C18 Column (250 mm × 4.6 mm, 5μm) with Mobile Phase consisting of Acetonitrile: Methanol: water (60:20:20 %v/v/v) at Flow Rate of 1 ml/min, injection volume 20 ml/min with UV detection at 223 nm. The method was validated for Linearity, Accuracy, Precision, Robustness, Specificity and system suitability parameters. The method was found to be linear over the concentration range of 8-40 μg/ml for telmisartan and 5-25 μg/ml for captopril with coefficient R2 0.9992 and 0.9994 respectively. Therefore, proposed method can be successfully used for routine analysis of telmisartan and captopril in bulk as well as synthetic mixture.

Keywords: Analytical method, Telmisartan, Captopril, UV, HPLC, RP-HPLC, HPTLC, UHPLC