Abstract

IN VITRO ASSESSMENT OF FREE RADICAL SCAVENGING AND HEPATOPROTECTIVE ACTIVITY OF ETHANOLIC LEAVES EXTRACT OF SPHAGNETICOLA TRILOBATA

Dr. P. Anitha* and R.G. Nithya Shri

ABSTRACT

Sphagneticola trilobata belonging to the family Asteraceae is a traditional medicinal plant associated with various bioactivities such as antioxidant, antibacterial, anti-inflames, antimalarial, antifungals, hepatoprotective, antidiabetic and antitumor reported by many literatures. In the present study, we examine the ethanolic leaf extract of the plant was evaluated for its in-vitro anti-oxidant potential by DPPH free radical scavenging activity and Nitric oxide scavenging activity using ascorbic acid as the standard. The extract exhibited antioxidant activity possessing IC50 values of 76.12 (DPPH Free Radical Scavenging Activity) and 68.02 (Nitric Oxide Scavenging Activity), in vitro bioassays are important in the evaluation of Sphagneticola trilobata to evaluate possible hepatoprotective effects. The aims of this study were to evaluate the pre-treatment of HepG2 cells with hepatoprotective agents against the damage induced by carbon tetrachloride (CCl4) and its Cytotoxicity was assessed using MTT and Trypan blue assays. Both assays revealed that the Sphagneticola trilobata extract reduced the cell viability of HepG2 cancer cells with IC50 value of 164.77μg/ml (MTT assay) and 227.08μg/ml (trypan blue assay). The observed effect substantiated that ethanolic leaves extract of Sphagneticola trilobata possess free radical scavenging and hepatoprotective activity. 7

Keywords: Hepatoprotective, free radical scavenging, HepG2 cell line, Sphagneticola trilobata.