Abstract

EVALUATION OF ANTIMICROBIAL DRESSINGS FOR IMPROVING WOUND HEALING THROUGH THE MUPIROCIN-IN-LIPOSOMES-IN-HYDROGEL DELIVERY SYSTEM

Brijesh Chaudhary*, Prabhudutta Panda, Balram Singh and Kanchan Mishra

ABSTRACT

We assessed the mupirocin-in-liposomes-in-hydrogel delivery system cytotoxicity, ability to stop the growth of biofilms, ability to affect mature biofilms, and lastly its promise as a wound therapy in an in vivo mouse burn model. The system was discovered to be harmless to keratinocytes, or HaCaT cells. As demonstrated by the resazurin and crystal violet assays, it was safe to use and demonstrated antibiofilm action against S. aureus biofilms, however the activity was more pronounced against planktonic bacteria and before biofilm formation than against mature biofilms. The biological potential of the system and the healing of burns observed over a 28-day period were assessed using an in vivo mouse burn model. According to in vivo research, the administration mechanism is just as effective as the marketed form of mupirocin at promoting wound healing. Histological analysis revealed no differences in the quality of the scar tissue that had healed, but the new delivery system's healing time was quicker than that of the marketed product. For a thorough review, additional animal experiments and the creation of more complex in vivo models are required.

Keywords: Mupirocin Liposomes Hydrogel, Biofilm, Wound, Keratinocytes.