Abstract

SYNERGISTIC IMMUNOSTIMULATORY ACTIVITY OF Terminalia bellerica GUM POLYSACCHARIDE WITH LEVAMISOLE

Das Biswajit *, Dash Suvakanta, Choudhury Ramesh Chandra,Chakraborty Jashobir

ABSTRACT

The present investigation deals with the immunosynergistic activity of Terminalia bellerica gum polysaccharide (TBGP) in combination with drug levamisole on experimental model. Cellular immunity was carried out by neutrophil adhesion test and carbon clearance assay, whereas, humoral immunity was analyzed by Haemagglutination antibody (HA) titer and SRBC-Induced Delayed-type hypersensitivity (DTH) response. Individual dose of TBGP was selected by Stair case method (up and down) and administered at 100, 500 & 1000 mg/kg orally and in combination with levamisole- 50 respectively. The established “EXTRAIMMUNE” tablet was used as positive control and immunosuppressant Cyclophosphamide (100mg/kg/day, p.o) used as negative control. TBGP at 500 and 1000 mg/kg produced significant increases in adhesion of neutrophils and an increase in phagocytic index in carbon clearance assay whereas combination of both shows more significant result. In case of the Effect of TBGP and TBGP in combination of Levamisole on humoral response influence on sheep erythrocyte specific HA titer in mice, cyclophosphamide showed significant inhibition in antibody titer response, while TBGP and specially TBGP with levamisole shows enhanced humoral responses.

Keywords: Terminalia bellerica gum polysaccharide (TBGP), Delayed-type hypersensitivity (DTH), Cyclophosphamide