Abstract

APPLICATION OF CASSIA AURICULATA FLOWER AND BACILLUS COAGULANS (MTCC8126) EXTRACTS AGAINST MULTIDRUG CTX GENE RESISTANT SALMONELLA TYPHI

Padmavathi N.*, Srinivetha A. S., Swetha S., Vijayan R.* and Ragunathan R.

ABSTRACT

In present day scenario, existing antibiotics are effective against S. typhi but the emergence of multidrug resistant (MDR) S. typhi against third-generation cephalosporins and fluoroquinolones constitute a major threat to the society. Salmonella typhi was tested for resistance against various antibiotics by Antibiotic Sensitivity Test (ABST). The DNA from S. typhi and plasmid from E. coli were isolated and run through Agarose gel electrophoresis and finally amplified using Polymerase Chain Reaction (PCR). In the present study plant based nano emulsion and microbial based products were prepared against multi drug resistant (MDR) Salmonella typhi bacterial pathogen. The Bacillus coagulans crude extract and Cassia auriculata nano emulsion were prepared. Thin layer chromatography (TLC), UV-Visible spectrophotometry, Fourier Transform Infrared (FTIR) were performed to characterize the extracted compounds from both flower and bacteria. Antibacterial activity against Salmonella typhi was determined for flower extract, nano emulsion and bacterial extracts using well diffusion method.

Keywords: Salmonella typhi, multidrug resistant, CTX, nano emulsion, Cassia auriculata, Bacillus coagulans.