Abstract

INVITRO ANTIOXIDANT ACTIVITY, TOTAL FLAVONOID AND TOTAL PHENOL CONTENT OF ETHANOLIC EXTRACT OF DIOSPYROS MELANOXYLON LEAVES

Sushma Mondi*, Koganti Prasad and Sujatha Doddala

ABSTRACT

The objective of the study was to assess the In vitro antioxidant activity of the ethanolic extract of Diospyros mealnoxylon leaves by ABTS [2,2-azino-bis (3-ethylbenzothiazoline- 6- sulfonic acid)] assay, DPPH (2,2-diphenyl picryl hydrazyl), metal chelating assay, total antioxidant activity by Phospho molybdenum method and reducing power assay. Phyto constituent’s flavonoid and phenol content are also assessed considering quercetin and gallic acid as the standards. Antioxidants are compounds that eliminate oxidative stress in biological systems. Oxidative stress is caused by various radicals formed in the system as a result of oxygen entering the biological system. The radical ions formed as a result of oxidation is removed from the system without damaging the biological system with the effect of antioxidants. The total flavonoid and total phenol content of ethanolic extract of Diospyros mealnoxylon leaves were established to be 301.67 ± 04.64 μg of quercetin/mg of dry extract and 140.00 ± 01.44 μg of gallic acid equivalents/mg of dry extract respectively. The antioxidant potential of the plant is proven by the ABTS and DPPH assay which can be explored as a therapeutic agent in free radical inducing disesaess.

Keywords: Antioxidant, Diospyros melanoxylon, Total phenol, Total flavonoid, Oxidative stress.