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REVIEW ARTICLE ON HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) METHOD DEVELOPMENT AND VALIDATION
Prince Kumar*
ABSTRACT Chromatographers face the need to develop a high-performance liquid chromatography (HPLC) method everyday. In this review systematic procedures for developing an HPLC method, based on the best information available. HPLC method development involves sample pre-treatment, choosing separation conditions, detection of sample bands, quantitation, and method validation. HPLC is an analytical tool which is able to detect, separate and quantify the drug, its various impurities and drug related degrades that can form on synthesis or storage. Validation of HPLC method gives information regarding various stages like Accuracy, specificity, linearity limit of detection, limit of quantification. Keywords: HPLC, mobile phase, stationary phase, method development, validation. [Download Article] [Download Certifiate] |