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World Journal of Pharmacy and
Pharmaceutical Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology

Impact Factor : 8.025

ICV : 84.65

WJPPS Citation

	All	Since 2019
Citation	5450	3969
h-index	23	20
i10-index	134	84

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Updated Version

WJPPS introducing updated version of OSTS (online submission and tracking system), which have dedicated control panel for both author and reviewer. Using this control panel author can submit manuscript

Call for Paper

WJPPS Invited to submit your valuable manuscripts for Coming Issue.

Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

New Impact Factor

WJPPS Impact Factor has been Increased to 8.025 for Year 2024.

WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

Scope Indexed

WJPPS is indexed in Scope Database based on the recommendation of the Content Selection Committee (CSC).

WJPPS: NOVEMBER ISSUE PUBLISHED

NOVEMBER 2024 Issue has been successfully launched on 1 NOVEMBER 2024.

Abstract

REVIEW ARTICLE ON HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) METHOD DEVELOPMENT AND VALIDATION

Prince Kumar*

ABSTRACT

Chromatographers face the need to develop a high-performance liquid chromatography (HPLC) method everyday. In this review systematic procedures for developing an HPLC method, based on the best information available. HPLC method development involves sample pre-treatment, choosing separation conditions, detection of sample bands, quantitation, and method validation. HPLC is an analytical tool which is able to detect, separate and quantify the drug, its various impurities and drug related degrades that can form on synthesis or storage. Validation of HPLC method gives information regarding various stages like Accuracy, specificity, linearity limit of detection, limit of quantification.

Keywords: HPLC, mobile phase, stationary phase, method development, validation.

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