Abstract

DEVELOPMENT AND VALIDATION OF RPHPLC METHOD FOR SIMULTANEOUS DETERMINATION OF CHLORPHENIRAMINE MALEATE AND PHENYLEPHRINE HYDROCHLORIDE IN LIQUID DOSAGE FORM

Priyanka P. Chirde*, A. P. Dewani and Dr. A. V. Chandewar

ABSTRACT

Chlorpheniramine maleate and Phenylephrine hydrochloride both are of antihistamine activity and utilised in numerous theraupaticprocedure. The present work describes development and validation of high-performance liquid chromatography -diode array detection (HPLC-DAD) procedure for analysis of Chlorpheniraminemaleate (CPM) and Phenylephrine hydrochloride (PHE) in syrup dosage form. Effective chromatographic separation of CPM and PHE was achieved using Hypersil gold C18 analytical column (4.6mm,150mm,5mm) with gradient elution of mobile phase composed of Orthophosphoric acid at the flow rate of 1.2 ml/min and the effluents were monitored at 268 nm. The retention time of CPM and PHE was found to be 2.9 min and 4.9 min respectively. The linearity for CPM and PHE was in the range of 0.5 -1.5µg/ml and 0.5 – 1.5 µg/ml with correlation coefficient of 0.996 and 0.964. The %recoveries of CPM and PHE was found to be 98.5% and 97.4%. The HPLC method was applied to the syrup dosage form in which analytes were successfully quantified with good recovery values, with no interfering peaks of excipients.

Keywords: Phenylephrine hydrochloride, Chlorpheniramine maleate, Orthophosphoric acid, Acetonitrile, HPLC- DAD.