Abstract

SCREENING, ISOLATION AND PRODUCTION OF ANTIBIOTICS FROM OIL CONTAMINATED TERRESTRIAL SOIL SAMPLE

Allada Lakshmi Tripu Kalyani*, Shaik Sasjida, Kondeti Sarvani, Dr. Y. A. Chowdary, P. Divya, Ullindla Chandrika, M. Sindhu

ABSTRACT

Actinomycetes represent a ubiquitous group of microbes widely distributed in natural ecosystem around the world and especially significant for their role in the recycling of organic matter. Actinomycetes are abundant in terrestrial soils, a source of most isolates shown to Produce bio active compounds. The main objective of the present investigation was to isolate and screen antibiotic producing actinomycetes from the soil samples of Krishna District. In the present work YEME (Yeast Extract malt Extract) media is employed for Selective isolation of Actinomycetes, Starch Casein agar medium was used for Maintenance and preservation of selective Actinomycetes isolates. In the Screening Program for antibiotic activity all the actinomycete’s are isolated by using spread plate method and to know the antimicrobial activity by using Cup Plate Method. Total 4 Actinomycetes colonies were isolated from OCS (Oil Contaminated Soil) Sample. These isolates were named as OCS-1 to OCS-4 then all the 4 isolates were Evaluated for antibiotic production for Antibiotic activity. Each isolate was Kept in special mediums for antibiotic Production for 7 days. For evaluation of Antibiotic activity by employing cup plate method using ampicillin as standard. After plotting graphs zone of inhibition was observed more in as ISP-3 compare to remaining. Out of 4 isolates 2 isolates OCS-1 and OCS-2 showed antibiotic production when we kept these for antibiotic Production.

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