Abstract

HPTLC METHOD DEVELOPMENT AND VALIDATION FOR METOPROLOL SUCCINATE AND OLMESARTAN MEDOXOMIL IN TABLET DOSAGE FORM

Tabassum I. Hangad*, Oais Shaikh, Mohib Isani, Dawood Godikar, Sakshi Katke and Aafreen Malvankar

ABSTRACT

Chromatographic separation studies were carried out on the working standard solution of Olmesartan Medoxomil and Metoprolol Succinate. Initially, trials were carried out using various solvents, to obtain the desired system suitability parameters. After few trials, Toluene: Ethyl acetate: Glacial acetic acid in proportion 6: 2.5: 0.5 (v/v/v) was chosen as the mobile phase for analysis. Other chromatographic conditions like chamber saturation time, run length, sample application volume, sample application positions, distance between tracks, detection wavelength, were optimized to give reproducible Rf values and symmetrical peak shape for the drug peak.

Keywords: High Performance Thin Layer Chromatography (HPTLC), Metoprolol Succinate, Olmesartan Medoxomil, Thin Layer Chromatography (TLC), Densitogram, Detection wavelength, Retention factor.