Abstract

NEW METHOD FOR SPECTROPHOTOMETRIC DETERMINATION OF CILOSTAZOL IN PURE FORM AND IN PHARMACEUTICAL FORMULATIONS

Malek Okdeh* and Leen Ghazali

ABSTRACT

An accurate, simple, fast and cheap spectrophotometric method has been developed for the determination of Cilostazol (CLS) in pharmaceutical pure and dosage forms. The method is based on the reaction of 2,6 Dichloro phenol indophenol (DCPIP) with Cilostazol in the presence of methyl alcohol. This reaction produces a complex orange colored product which absorbs maximally at 445 nm. Beer’s law was obeyed in the range of 3.35 - 77.25 μg/mL with molar absorptivity of 4.390×103 L. mole-1 cm-1 Sandell’s sensitivity 0.0842 μg.cm-2. The effects of variables such as temperature, heating time, concentration of color producing reagent, and stability of color were investigated to optimize the procedure. The results are validated statistically. The proposed method was applied to commercially available tablets, and the results were Pharmaceutical formulations.

Keywords: Cilostazol; 2,6 Dichloro phenol indophenol; Spectrophotometry; Beer’s law complex.