Abstract

UTILIZING GOLD NANORODS APTAMER-BASED COLORIMETRIC DETECTION OF DOPAMINE IN THE BIOLOGICAL SAMPLES

Ghadah Rashed Ghazi Alotaibi*

ABSTRACT

The extracellular fluid of the central nervous system contains dopamine in quantities ranging from nanomolar to micromolar (10 nM to 1 M). The concentration of dopamine has been routinely evaluated by medical researchers utilizing traditional chromatography, capillary electrophoresis, enzyme assay, and rapid liquid chromatography/tandem mass spectrometry (LC-MS/MS) techniques because of the substance's physiological significance. However, the majority of these techniques take a long time, need for expensive equipment, specialised training, and intricate pre-treatment procedures, have little reproducibility, and require expensive equipment and training. Determining the trace amount of dopamine in a biological sample requires the development of a cheap, quick, and accurate approach, which is currently a very difficult task. Colorimetric methods are convenient and simple to monitor with the naked eye since they don't require any costly equipment. Using a colorimetric aptasensor based on gold nanorods, the detection of dopamine was demonstrated in this paper. The developed sensor avoided interference from ascorbic acid and displayed good sensitivity and selectivity towards dopamine. Good linear range (0.1 μM-100 μM) was demonstrated by this colorimetric aptasensor. Gold nanorods are an excellent method for detection since dopamine may be detected at concentrations as low as 0.1 μM because it is only found in extremely tiny amounts in human bodies. As a result of AuNRs having a longitudinal axis, which increases their sensitivity to even the smallest environmental change, detection is made easier and more straightforward.

Keywords: Dopamine, Physiological importance, Detection techniques, Gold nanorods, and Colorimetric aptasensor.