Abstract

U.V. AND HPLC METHOD FOR THE ESTIMATION OF DEFLAZACORT IN MARKETED FORMULATION

Pooja Malviya* and Paliwal

ABSTRACT

The objective of present research work is U.V. and HPLC method For the estimation of deflazacort in marketed formulation. Deflazacort (DFZ) is a glucocorticoid used as an anti-inflammatory and immunosuppressant drug. The objective of this study was to develop, validate and compare UV spectrophotometric and highperformance liquid chromatography methods, for the quantitative determination of DFZ in tablets. The parameters linearity, accuracy, precision, limit of detection limit of quantitation, range were studied as per International Conference on Harmonization guidelines. UV spectroscopic determination was carried out at an absorption maximum of 242 nm nm using 0.1N NaoH as solvent. Results of the analyses were validated statistically and by recovery studies. The proposed method is simple, rapid, precise and accurate and can be used for the reliable quantitation of deflazacort in pharmaceutical formulation. In the UV spectroscopic method linearity over the concentration range of deflazacort was found to be 5-25ug/ml with a correlation coefficient 0.998.

Keywords: Linearity, precision, accuracy, robustness, validation.