Abstract

ANTIBACTERIAL EFFECT OF CIPROFLOXACIN MICROEMULSION LOADED WITH TEA TREE OIL

Mamatha G.T., Pavan Kumar* and Mahendra Kumar M. S.

ABSTRACT

The present investigation was aimed to study the antibacterial effect of Ciprofloxacin hydrochloride along with Tea tree oil to be prepared as microemulsion and explore the better antibacterial effect. Microemulsions were prepared by titrating different ratio of oil to Smix (Surfactant + Co-surfactant) with water and microemulsion region was recorded in the Pseudoternary phase diagram. Microemulsion preparations were characterized for compatibility, transmittance, viscosity, pH, % drug content, surface morphology, globule size and zeta potential, In vitrodrug release and stability studies. The microemulsion gel was prepared for optimized microemulsion preparation TCM3 by incorporated into 1% w/w Carbopol gel and evaluated for various parameters like spreadability, viscosity, pH, drug content, In vitro drug release and the In vitro Antibacterial effect was carried out for the drug, oils, TCM3 and TCM3-G1 preparations. Prepared microemulsion and microemulsion gel were evaluated for various parameters which showed better results. In vitro antibacterial study confirmed that when Ciprofloxacin hydrochloride is combined with Tea tree oil in the form of microemulsion have shown synergistic Antibacterial effect compared with drug and oil separately, Tea tree oil-based Ciprofloxacin hydrochloride microemulsion were prepared for topical application. TCM3 formulation taken as an optimized formulation because of high % transmittance, less viscous, high drug content and shows topical pH, more In vitro drug released and good stability. In vitro antibacterial effects studied on staphylococcus aureus bacterial strain. Microemulsion drug delivery system can improve therapeutic effect of the topical drugs and combination of drug and essential oils in the form of microemulsion showed synergistic activity and give better therapeutic effect for topical drug delivery.

Keywords: Microemulsions, Ciprofloxacin hydrochloride, Topical drug delivery, Tea tree oil.