Abstract

HISTOCHEMICAL ANALYSES OF THE NUCLEIC ACIDS, LIPIDS AND BOUND LIPIDS IN THE SECRETORY DYNAMICS OF THE NEUROSECRETORY MATERIALS OF EARTHWORM, METAPHIRE PEGUANA CORRESPOND TO THE DIFFERENT SEASONS

Dr. Trijit Nanda* and Dr. Rani Nanda

ABSTRACT

Impact of seasonal temperature variations on the cytomorphic alterations of the neurosecretory cells of both the ganglia in M. peguana reveal that the volume of the nuclei declines appreciably and the neurosecretory substance remain stored within the perikarya during the successive seasons. The morphological activation reaches the peak at the early monsoon period. However, during this period the larger neurosecretory cells show enlargement of nuclei and their perikarya. Besides spectacular engorgement of neurosecretory material within the neurosecretory cells are noticeable. So it can be speculated that winter season is the ideal breeding season of the species under study. The intensity of the neurosecretory materials accumulation at the neurohaemal sites in accordance with the seasonal variations both ganglia reveal the following criteria: At the pre- monsoon the neuropile do not show arborization of neurosecretory fibres but in some instances accumulation of the neurosecretory materials may be observed at the margin of the neuropile. The early monsoon period also maintains the same trends but at late monsoons rich vascularization are observed in both ganglia and neuropile show finer branches of capillaries and neurosecretory fibers possessing deep stainable substances. Axonal transport may be noticeable in confluence with the capillaries. At post monsoon period neurosecretory material accumulation is noticeable at the accumulation zone where capillaries containing histochemical stainable inclusions are prevalent.

Keywords: Neurosecretory Materials (NSM), Neurohaemal sites (NS), Neurosecretory Fibres(NF), Neurosecretory cells(NSCS).