Abstract

EVALUATION OF ANTIOXIDANT ACTIVITY OF DIFFERENT PARTS OF CAJANUS CAJAN (L.) BY USING IN-VITRO ASSAYS

Ravi Kumar* and Shipra Yadav

ABSTRACT

The present study was designed to investigate the antioxidant activity of different parts of Cajanus cajan (L.) by in-vitro assays. Antioxidant activity was performed by extracting various parts of the extract (leaf extract, flower extract, pod extract, dal extract, root extract) in various solvents (hexane, methanol, chloroform, ethyl acetate, and water) by the percolation method and antioxidant potential was also estimated by employing various antioxidant assays: 2, 2-diphenyl-1-picrylhydrazyl (DPPH), Nitric Oxide (NO), Ferric Reducing Power Assay (FRAP), Reducing Power Assay (RPA), Total Antioxidant Capacity (TAC), Total Flavonoid Determination (TFD) and Total Phenolic Determination (TPD). The SC, SE from the stem, LE (leaf ethyl acetate extract), LM (leaf methanol extract) from leaf, FC (flower chloroform extract), FM (flower methanol extract) from the flower, DW (dal water extract) from dal, and PE (pod ethyl acetate extract), PW (pod water extract) from pod showed good reducing potential, whereas others showed moderate activity. The extracts showed good radical scavenging potential. RM (root methanol extract), SE (stem ethyl acetate extract), LE, FH (flower hexane extract), DM, and PW showed the highest flavonoid content compared to other extracts. Cajanus cajan (L.) (Pigeon pea) is a potential source of antioxidants due to the antioxidant and free radical scavenging properties of its numerous components.

Keywords: Pigeon pea, Antioxidant Activity, DPPH, FRAP Assay, Free Radical Scavenging.