Abstract

CHARACTERIZATION OF LOADED LIPOSOMES

Pranav Jadahav*, Choure Sagar Goverdhan, Ashitosh Suresh Shahapure and Kamble Siddharth Sugandh

ABSTRACT

The examination of liposomes using traditional (SEC) and high performance (HPSEC) size discharge chromatography is the main topic of this paper. Regarding the field of liposome applications, the effectiveness of both procedures is evaluated. The use of an HPLC system coupled to gel columns with a size selectivity range permitting liposome characterisation in addition to particle separation significantly increases the potentiality of conventional SEC. Experimental aspects of size exclusion chromatography are reviewed, and a method based on HPSEC coupled to multidetection modes for on-line analysis of liposomes via label or substance encapsulation is offered. Applications of conventional SEC and HPSEC that deal with polydispersity, size and encapsulation stability, bilayer permeabilization, liposome production and reconstitution, and integration of amphiphilic compounds are presented. A straightforward and effective method for examining the encapsulation, insertion, and interaction of compounds from.

Keywords: High performance gel exclusion chromatography; Gel filtration; Liposome; Liposome reconstitution; Encapsulation; Permeability.