Abstract

EVALUATION ANTIBACTERIAL ACTIVITY AND GCMS ANALYSIS OF DATURA STRAMONIUM LEAF EXTRACT AGAINST BACTERIAL PATHOGENS

Smita Sabale*, Mohini Chaure, Isha Agnihotri and Sachin Laddha

ABSTRACT

Medicinal plants are used for the treatment of microbial diseases due to their valuable effects in healthcare. Plant based drugs are indeed the first material used as an alternative medicine against many diseases. The objective of this study is to determine the phytochemical constituent and antibacterial activity of Datura stramonium leaf extract against bacterial pathogen causing nosocomial infection. The leaf extracts of D. stramonium were prepared by Continuous Soxhlet extraction method. Antibacterial activity of D. stramonium leaf extracts against Staphylococcus aureus, Salmonella typhi, Escherichia coli, Pseudomonas aeruginosa were studied by agar well diffusion method. Antibacterial activity was present in the methanol extract of D.stramonium showed maximum antibacterial activity against Pseudomonas aeruginosa followed by Staphylococcus aureus. Phytochemical analysis showed presence of alkaloids, tannins and arthraquinones in extract. GCMS analysis of Datura stramonium leaf extracts reveled presence of Dibutyl phthalate, Neophytadiene and Benzyl benzoate. Phytochemical compounds present in this plant extract responsible for antibacterial effect on bacterial pathogens. This biological active compound shows therapeutic uses, reliability and less toxicity to treat various infection caused by this bacterial pathogens. The D.stramonium leaf extract possess pharmacological and physiological roles to develop plant based drug and application in pharmaceutical industries.

Keywords: Antibacterial activity, Datura stramonium, GCMS analysis, Staphylococcus aureus, Pseudomonas aeruginosa.