Abstract

BIOCHEMICAL AND HISTOPATHOLOGICAL ALTERATIONS RENDERED BY COMBINATION INSECTICIDE (CHLOROPYRIFOS + CYPERMETHRIN) IN TWO GENERATIONS OF GALLUS DOMESTICUS

*Asiya N. Khan

ABSTRACT

Effects of in-ovo intoxication of an insecticide formulation Nurelle D 505 EC (chlorpyrifos 50% EC + cypermethrin 5% EC) was evaluated in two generations of domestic fowl. The investigation covered four groups of fertilized RIR eggs - three for experimental and one for control. Experimental groups of eggs received doses of 0.01, 0.05 and 0.1 μg/egg of combination insecticide (Ci) and the control received corn oil on day zero of incubation. The doses were selected based on our previous study. The eggs were incubated and upon hatching the F1 generation chicks were sorted as per their treatment, tagged and reared on standard diet. Upon sexual maturity the birds from the same groups were allowed pen mating. The fertilized eggs were collected and incubated for the next generation (F2). Blood samples were drawn from the brachial vein of 25 weeks old parent F1 generation and 4 weeks old chick of F2 generation to evaluate biochemical parameters which included Serum glucose, Serum albumin, Serum globulin, Serum protein, Urea, Blood Urea Nitrogen (BUN), Creatinine, Alkaline phosphatase (ALP), Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and nucleic acid (DNA, RNA) content. Also The control and treated birds belonging to F1 and F2 generations were terminally sacrificed at the age of 25 weeks and 4 weeks respectively. Liver and kidney of each bird were processed for histopathological examination. The study showed that results of the biochemical and histopathological tests in the F1 and F2 generations, significant variations were found in the Ci treated group when compared to the control group of birds. This is the first report of Ci induced biochemical and histopathological alteration in the second generation of chicks and we believe that the test system can evolve as an alternate model for testing two generation developmental toxicity.

Keywords: Biochemical and histopathological alterations, combination insecticide (chloropyrifos + cypermethrin), two generations of gallus domesticus, gallus domesticus.