Abstract

HPTLC FINGERPRINTING STUDY OF HYDROALCOHOLIC EXTRACT FOR TANNINS FROM Ailanthus excelsa (Roxb.) LEAVES

Mudit Kumar*, Amita Tilak and Sudhir Singh Gangwar

ABSTRACT

Objective: The present study was conducted to identify the tannins from Hydroalcohol extract of medicinally and economically useful leaves of Ailanthus excelsam (Roxb.) using High Performance Thin Layer Chromatography (HPTLC) technique. Methods: Preliminary phytochemical screening was done and HPTLC studies were carried out. CAMAG HPTLC system equipped with Linomat V applicator (Switzerland). Densitometric scanning was performed with Camag TLC scanner IV in the reflectance absorbance mode at 540 nm and operated by Win CATS software (1.4.6 Camag) with the help of tungstant lamp. Results: HPTLC finger printing of tannins of Hydroalcohol extract of leaves shows absence of tannins as no dark blue color bands are seen after derivatisation with iron chloride. Conclusions: With the results of HPTLC analysis we have concluded the absence of tannins in the extract.

Keywords: Ailanthus excelsa (Roxb.) leaves, tannins, HPTLC Fingerprinting.