Abstract

DESIGN, DEVELOPMENT AND CHARACTERIZATION OF CORE-MODIFIED NANOLIPOIDAL CARRIER FOR DELIEVRY OF DOCETAXEL

Girja*, Twinkle Garg and R. B. Sharma

ABSTRACT

NLCs (Nanostructured lipid carriers) are one of the most promising nano-carriers for the development of efficient cancer chemotherapeutics targeted therapy. The core matrix of these biocompatible and/or biodegradable lipid-based nanoparticles is made up of solid and liquid lipids dispersed in a surfactant solution. Docetaxel is an anti-neoplastic agent used in the treatment process of various types of cancers including ovarian cancer, prostate cancer, small cell lung cancer, breast cancer, head/neck cancer and non-small cell lung cancer. The nanostructured lipid carriers of docetaxel were developed to offer instant drug release for rapid action during tumour formation and expansion. The prepared nanocarriers were characterized for drug loading and drug entrapment, particle size, zeta potential and drug release profile. The nanoparticles were evaluated for their potential by using MTT assay. These studies revealed the successful formulation of the NLCs. Micromeritics data revealed that the NLCs were the in nanometric range with a narrow polydispersity. Morphological study was done by TEM analysis, which revealed the spherical and the non-aggregated nature. In-vitro release studies helped in finding the profile and order kinetics of prepared formulation. In-vitro cytotoxicity assay showed that formulation has maximum anti-cancer activity vis-à-vis plain drug.

Keywords: Nanostructured lipid, NLC, anti-cancer drugs, Docetaxel, Cytotoxicity assay.