Abstract

MOLECULAR DOCKING, SYNTHESIS AND IN-VITRO ANTIBACTERIAL ACTIVITY OF ISATIN CONTAINING SCHIFF BASE DERIVATIVES

Parvathi S.*, Umaparvathy S., Ramya R. and Mohanapriya G.V.

ABSTRACT

The schiff base derivatives reported to contain anti microbial, anti HIV, anti inflammatory, antitubercular, anti sars and anti cancer activity in many research works. A series of isatin-schiff base derivatives having a great anti-bacterial and anti- fungal activity are designed using the chem-sketch software. A series of twelve isatin-Schiff base derivatives generated in-silico is subjected to docking studies with a filamentous temperature sensitive Z protein, which is a bacterial protein used in the initial stages of the bacterial replication. The isatin-schiff base derivatives dock in the protein and inhibits the bacterial replication. The binding energies of the series of isatin-schiff base derivatives with the bacterial protein are obtained and one among the derivatives with greater binding energy(derivative 11) is selected and synthesized. The synthesized drug is tested for invitro activity by Kirby Bauer test using Staphylococcus aureus culture and the zone of inhibition is measured. The ADMET activity of all the twelve derivative of the isatin-schiff base derivatives are determined using SWISS ADMET software.

Keywords: Isatin-schiff base derivative, filamentous temperature sensitive Z protein, bacterial replication, binding energy, Kirby Bauer method, Staphylococcus aureus, ADMET activity.