Abstract

SIMULTANEOUS EQUATION METHOD FOR THE ESTIMATION OF CETIRIZINE HYDROCHLORIDE AND GRISEOFULVIN BY UV SPECTROPHOTOMETRY

Harshvardhan T. Suryawanshi*, Ashpak M. Tamboli, Rohan T. Kshirsagar and Hasan I. Shaikhmulani

ABSTRACT

The simple, accurate, and precise UV spectrophotometric method has been developed for simultaneous estimation of Cetirizine Hydrochloride and Griseofulvin in bulk tablet formulation. Two wavelength 237 nm and 292 nm were selected for estimation of Cetirizine Hydrochloride and Griseofulvin by simultaneous equation method respectively. UV spectrophotometric method was developed as per ICH guideline using Methanol and Water as a Mobile phase. Cetirizine Hydrochloride and Griseofulvin individually follows the Beer-Lamberts Law over concentration range 0.5-2.5 & 12-62 μg/mL, regression of coefficient was found to be r2=0.997 & r2=0.998 respectively. The percentage recovery was found to be 93 to 101% at three different levels. The proposed method was successfully applied for determination of Cetirizine Hydrochloride and Griseofulvin in tablet dosage form as per ICH guideline the result of the analysis were validated statistically and were found to be satisfactory.

Keywords: Cetirizine Hydrochloride, Griseofulvin, Simultaneous equation, UV spectrophotometer, validation.