Abstract

COMPARISON OF THE FUNCTIONING OF CHICKEN ILEUM TO RAT ILEUM AND ASSESSING ITS REPRODUCIBILITY AND ACCURACY FOR DOSE RESPONSE CURVES - A TOOL TO AID POST GRADUATE ANIMAL EXPERIMENTATION TRAINING

Gauri Mittal* and Sunita Mittal

ABSTRACT

Introduction: As per the compendium of Committee for the purpose of control and supervision of experiments on animals (CPCSEA) 2018, one of the objectives of the Institutional Animals Ethics Committee (IAEC) is to ensure that „As far as possible, experiments are not performed merely for the purpose of acquiring manual skill‟. Chicken gut tissue sourced from butcher shops which is a waste material is one of the alternatives which can be used for initial training and even afterwards to prevent sacrificing an animal for the sole purpose of the experiment. Objective: The aim of this study was to compare the functioning of rat ileum to chicken ileum and assess its reproducibility and accuracy. Methodology: The dose response curve (DRC) of chicken ileum tissue was compared to rat ileum tissue with Acetylcholine (Ach) solution at multiple similar concentrations in the presence of a similar physiological salt solution (PSS). Results: Log dose-response curves were sigmoid for both rat and chicken ileum. Conclusion: Chicken ileum can be used as an alternative to rat ileum in bioassay, in the absence of an animal house as well as in the presence of one, to train students in the initial period of post-graduation.

Keywords: Chicken ileum, Rat ileum, Animal experimentation, Post graduate training, dose response curve.