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Abstract

HIGH - PERFORMANCE LIQUID CHROMATOGRAPHY –AN IN-DEPTH OVERVIEW

Dipti Sambhaji Patil, Tejaswini Raju Kande*, Tanmayi Dattatray Kalamkar, Naziya Moulali Sayyad and Supriya Sopan Khatal

ABSTRACT

HPLC (High Performance Liquid chromatography) is an analytical technique widely used for identification, separation, detection and quantification of various drugs and its related degradants. Appropriate mobile phase, stationary phase, column, column size, temperature, wavelength and gradient selection is important for the suitable compatibility and stability of drug as well as impurities and degradants.[1] HPLC method is versatile because of the several advantages like rapidity, specificity, accuracy, precision and ease of automation included in this method.

Keywords: HPLC, Analysis, Validation, Detection, Identification, Quantitation.


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