Abstract

AN OVERVIEW – BIOANALYTICAL TECHNIQUES AND ITS APPLICATIONS

Mokshada Kulal*, Santosh Jadhav and Monali Shingade

ABSTRACT

Bioanalysis refers to the quantification of chemicals or drug substances, as well as their metabolic products, in a wide range of bio-samples. The advancement of bioanalytical techniques has resulted in a progressive discipline with many exciting opportunities for future advancement. In the pharmaceutical industry, the main impact of bioanalysis is to obtain a quantitative measure of the drug and its metabolites. The goal is to conduct pharmacokinetic / pharmacodynamic studies on pharmacokinetics, toxicokinetics, bioequivalence, and exposure response. Various bioanalytical techniques, such as hyphenated techniques, chromatographic techniques, and ligand binding assays, are used in bioanalytical studies. This review emphasises the importance of bioanalytical techniques and hyphenated instruments in evaluating drug bioanalysis. Various bioanalytical techniques used for the quantitative estimation of drugs and their metabolites in biological matrices, such as liquid-liquid extraction, solid phase extraction, high performance liquid chromatography, gas chromatography, gas chromatography-mass spectroscopy, liquid chromatography-mass spectroscopy, capillary electrophoresis-mass spectroscopy, and protein precipitation, were critically highlighted in this review.

Keywords: Bioanalysis, Hyphenated technique, Pharmacokinetics, Bioequivalence, chromatography.