Abstract

PLATELET-RICH PLASMA AND GINGER EXTRACT MITIGATE TOXICITY AND OXIDATIVE STRESS MOTIVATED BY PARACETAMOL IN LIVER OF MALE RATS

Manar Nhaito, Amera Sayed, Amina Shwata, Asmaa EL-madany, Maha Reda, Yasmine Farrag and Asmaa Magdy Zaazaa*

ABSTRACT

Objective: The present study aimed to evaluate the protective effect of Platelet-rich plasma (PRP) and ginger extract (GE) on oxidative stress, inflammatory markers, apoptotic and antiapoptotic markers in the liver tissue of the paracetamol treated rats. Methods: Forty male Wistar rats were divided into five groups; Group (1): Negative control group (Con), Group(2): Positive control group orally received paracetamol (PCM) in a single oral dose of 3 gm/kg b. wt., Group(3): PRP and PCM treated group orally administered 3 gm/kg b. wt. of PCM and treated with PRP intraperitoneal by single dose 50 μL (PCM+PRP), Group (4): GE and PCM treated group orally administered 3 gm/kg b. wt. of PCM and treated with GE 250 mg/kg of BW/day for four weeks (PCM+GE) and Group (5): Rats were injected orally with a single oral dose of paracetamol at 3 g/kg then treated with both of PRP and GE at the same previous doses. Results: PCM exposure resulted in significant elevations of oxidative stress, as evidenced by the increased malondialdehyde level associated with significant decrease in glutathione reduced and glutathione peroxidase activity in the liver tissue. Moreover, PCM caused an up regulation in the values of liver function enzymes. PRP and GE significantly attenuated the PCM-evoked liver oxidative stress and modulated the activities of liver function enzymes. Histopathological examination of liver tissue showed degeneration in the hepatocytes associated with inflammatory cells penetration in portal zone in PCM group. Meanwhile, the treatment of PCM groups with PRP and GE were found to restore the structural organization of the liver. Conclusion: These data suggest that PRP and GE protect rat liver from PC-induced oxidative stress, probably via its antioxidant activity. So, PRP and GE are promising pharmacological agents for preventing the potential hepatotoxicity of PCM.

Keywords: Paracetamol, PRP, Ginger, Hepatotoxicity, Antioxidant.