Abstract

UV VISIBLE SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION FOR DETERMINATION OF PAZOPANIB HYDROCHLORIDE IN BULK AND TABLET DOSAGE FORM

Rupali Khairate*, Gazala Patel and Smita Kumbhar

ABSTRACT

A new simple, accurate, sensitive, and precise uv visible Spectrophotometric method was developed and validated for the determination of Pazopanib hydrochloride in bulk and tablet dosage form. The Pazopanib hydrochloride showed the maximum absorbance at 271 nm in uv range (200-400 nm). The developed method was proved linear in the range of 5-25 μg/ml and displayed good regression coefficient (R2 ꞊ 0.998). The % RSD for interday precision and intraday precision was 0.087 and 0.188 respectively. The LOD and LOQ found to be 1.2187 μg/ml and 3.6932 μg/ml respectively. The proposed method was validated for linearity, accuracy, precision, sensitivity and robustness as per ICH guidelines. The results obtained shows that the developed method can be utilized for routine analysis of Pazopanib hydrochloride in bulk and tablet dosage form.

Keywords: Pazopanib hydrochloride, Ethanol, Uv spectrophotometric method, Method Development.