Abstract

A REVIEW ON HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

Bhagyashri V. Bangar* and Narendra M. Gowekar

ABSTRACT

The term HPLC stands for high- performance liquid chromatography. The most versatile and extensively used type of elution chromatography is high-performance liquid chromatography (HPLC). Scientists utilise the method to separate and identify species in organic, inorganic, and biological materials. The mobile phase is a liquid solvent that contains the sample as a combination of solutes in liquid chromatography. Separation mechanisms and stationary phase types are frequently used to categorize HPLC.[1] partition chromatography, or liquid- liquid chromatography.[2] adsorption chromatography, or liquid-solid chromatography.[3] ion- exchange chromatography, or ion chromatography.[4] size-exclusion chromatography.[5] affinity chromatography, and[6] chiral chromatography re the different types. Chromatography is a separating method. High-different liquid chromatography (HPLC) is an important method for analyzing drugs, both qualitatively and quantitatively. The amount of compounding coimpound in a solution can be determined using HPLC. This article describes the review of HPLC including principals, Types, instrumentation, and application. HPLC is an analytical tool that can identify, quantify the various drug, as well as their different impurities and drug-related degradants that can form in storage manufacture to detect and quantify qualitatively drug-related impurities that may be introduced during synthesis.

Keywords: HPLC, Principal, Types, Instrumentation, Application.