Abstract

MICROBIOME IN CATHARANTHUS ROSEUS LEAVES FOR PHARMACEUTICAL DEVELOPMENT

Nguyen Hoang Khue Tu*, Nguyen Pham Quynh Anh, Doan Thi Thanh Vinh, Doan Thi Thanh Tra, Vo Duy Le Giang, Nguyen Ngoc Huu and Santa Romero Jovel

ABSTRACT

Catharanthus roseus is well-known plant that contained many valuable polyphenols and indole alkaloids with the biological activities such as anticancer, anti-diabetes, anti-hypertension activities. Finding and exploring endophytic microbiome in Catharanthus roseus are important to know the correlation of microbial community and plant. Next-Generation Sequencing (NGS) contributes fast analysis of uncultured microbial community for evolution and functional development. By using the high throughput techniques of NGS, the 16S rRNA sequences (V1–V9 regions) were determined with the Illumina metagenomics technique to generate a total of 691,248 reads from the leaves. The leaf sample had Proteobacteria, Firmicus, Actinobateriota, Deinococcota, Chloroflexi without Myxocota. The predicted function of metagenomics by using MetaCyc database was obtained. The distribution rates were calculated and estimated by PICRUSt2. The study found many functional pathways, especially the markers of gallate derivative degradation, superpathway of chorismate metabolism, superpathway of S-adenosyl-L-methionine biosynthesis, polyisoprenoid biosynthesis (E. coli), superpathway of geranylgeranyl diphosphate biosynthesis (II), mevalonate pathway (I), gallate degradation (I,II), involving polyphenol (gallic acid) and indole alkaloid synthesis in Catharanthus roseus. The study suggested the importance of metagenomics to be applied into pharmaceutical field and life science.

Keywords: Catharanthus roseus leaves, biological activities, bacterial diversity; Next-Generation Sequencing.