Abstract

UV SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF LINAGLIPTIN IN BULK AND TABLET DOSAGE FORM

Amruta Dalal*, Dr. Varsha Tegeli and Rajashri Waghmode

ABSTRACT

Objective: The objective of the present work is to develop a new, simple, economical, precise, sensitive, linear, accurate, rapid UV Spectrophotometeric method has been developed for the estimation of Linagliptin in bulk and pharmaceutical formulation as per ICH guidelines. Method: Spiked Linagliptin arrangement was checked over UV- visible extend for its wavelength of greatest absorbance. Results: The wavelength of most extreme absorbance for Linagliptin was found to be 296 nm. The relationship coefficient over the concentration extend of 40-115μg/ml was found to be 0.9993. The LOD and LOQ of Linagliptin was found to be 2.7039 and 8.1938 respectively. The method was successfully applied to Linagliptin in marketed formulation ands result were in good agreement with label claims. Conclusion: Depending on the results, the given method can be successfully applied of Linagliptin in Tablet formulation.

Keywords: Linagliptin, Ethanol, UV –Visible Spectrophotometric method, Development, Validation.