Abstract

ISOLATION AND STUDY OF PSYCHROTROPHIC Enterobacter hormaechei SSwC2 HYDROLYTIC ENZYMES

Syed Nisar Ahmed*

ABSTRACT

Psychrotrophic bacteria have been extensively isolated from milk and dairy products such as Staphylococcus spp., Pseudomonas spp., Enterobacter spp., Bacillus spp., Acinetobacter spp., Alcaligenes spp., Aeromonas spp., Vibrio spp., Flavobacterium spp., Micrococcus spp. and Achromobacter spp. Ibrahim et al., (2005). The psychrotrophic bacteria may have direct as well as an indirect effect on the quality of milk and dairy products (Bishop et al., 1994). Metabolic processes in psychrophiles and psychrotrophs have been found to be heat labile Mitchell et al., (1985) and Ochiai et al., (1979). Study of enzymes from cold-adapted microorganisms may provide novel ways of understanding thermo biochemistry as well as lead to possible applications of such enzymes in the processing of food and the biotransformation of chemicals at low temperature. In this present work psychrotrophic bacteria Enterobacter hormaechei SSwC2 was isolated from sweet curd sample obtained from Seema milk dairy, Aurangabad, (MH), India. Molecular identification was done by 16S rRNA sequencing, partial sequence was submitted to NCBI GeneBank database and received accession number (MW412933). Proteolytic and lipolytic activity of Enterobacter hormaechei SSwC2 was carried out. Bacterium showed maximum proteolytic activity as a selection ratio of 5.2 at 200C whereas lowest at 450C i.e. 3.1 respectively. At 70C Enterobacter hormaechei SSwC2 showed selection ratio of 4.4. Similarly Selection Ratio (S: R value) at different pH was measured as maximum 5.2 S: R value at pH 8 whereas lowest at pH 10 i.e. 2.4 S: R value at optimum temperature conditions. Enterobacter hormaechei SSwC2 does not showed lipolytic activity.

Keywords: Psychrotrophs, Enterobacter hormaechei, protease, wash performance, reflectance.