Abstract

DEVELOPMENT OF A RAPID SEPARATION PROCESS FOR CURCUMIN FROM CURCUMA LONGA L. RHIZOMES AND ITS QUANTIFICATION BY HPLC-PDA

*Subash Chandra Verma

ABSTRACT

In the present work, a shortest and rapid separation process for curcumin from Curcuma longa rhizomes, using microwave-assisted extraction (MAE) technique, has been developed. The obtained extracts were analyzed by using HPLC coupled to photo diode array detector (PDA) to prove the MAE extraction efficiency. The optimal MAE conditions were dichloromethane as extracting solvent, at a temperature equal to 40°C for 3 min. Further, Curcumin was isolated from dichloromethane-MAE extract of C. longa rhizomes and characterized by using FTIR, NMR and EIMS techniques. MAE can be used as an alternative method for isolation of curcumin from C. longa rhizomes due to its efficiency and rapidity as compared to the conventional method with column chromatography. The developed procedure may be useful for researchers, academicians, pharmaceutical and cosmetic industries.

Keywords: Curcuma longa L., MAE, FTIR, NMR, curcumin.