Abstract

FORMULATION AND EVALUATION OF DARUNAVIR LOADED SOLID LIPID NANOPARTICLES

Dinesh Kumar Sandirigiri* and K. Anie Vijetha

ABSTRACT

Darunavir is an anti-retroviral drug and it is inhibitor of HIV protease with poor water solubility. So, the main objective of the study is to improve the solubility and bioavailability of poor water soluble drug Darunavir. The Present study outlines the formulation of the Darunavir loaded solid lipid nanoparticles, Particle size analysis, Surface morphology, dissolution studies, drug loading, entrapment efficiency and drug release kinetics along with mechanisms. In general, Solid lipid nanoparticles were prepared by using various methods like homogenization, ultrasonication. The Optimized Solid lipid nanoparticles formed with Darunavir resulted in particle size of 334 nm, Poly dispersity index (0.184), zeta potential (-12.75) which results in stable dispersion. In-vitro drug release studies in 6.8 PH phosphate buffer shown 91.2 % drug release at the last time period of 12 hrs and followed zero order release kinetics. Differential Scanning Calorimetry Studies were performed and found no chemical interactions. Surface morphology was studied by Scanning Electron Microscopy analysis and found that particles with spherical shape and smooth surface were appeared. Accelerated stability studies which was performed at 25.30 and 400 C temperatures after 3 months there was no change in the stability of dispersion upon storage conditions.

Keywords: Solid lipid Nanoparticles, surface morphology, accelerated stability studies, poly dispersity index, Scanning Electron Microscopy.