Abstract

EVALUATION OF ANTIDIABETIC AND HEPATOPROTECTIVE ACTIVITY OF METHANOLIC EXTRACT OF ALPINIA LEPTOSTACHYA HOOK

Mukkara Sukanya* and P. Dharani Prasad

ABSTRACT

To evaluate the antidiabetic and hepatoprotective activity of methanolic extract of Alpinia Leptostachya Hook (MEAL) in alloxan induced diabetic rats. A single dose of alloxan monohydrate (120mg/kg, i.p.) was used to induce diabetes mellitus. The MEAL at the dose of 200 and 400mg/kg and the standard drug glibenclamide (2.5mg/kg) were administered orally to the diabetic rats for 15 days. The FBG levels were estimated on 0, 5, 10 and 15th day. On 15th day, the blood was collected and serum is separated for the estimation of biochemical parameters and the pancreas was isolated for histopathological studies. In our investigation, the biochemical changes were observed after 36h of CCl4 treatment. Thereby, it was found that the animal groups which are pretreated with MEAL at the dose of 200 and 400mg/kg (groups-III and IV) as well as silymarin at the dose of 100mg/kg (group-V) for three times at 12h. intervals, resulted in significantly decreases the hepatic enzymes such as SGOT, SGPT, ALP and γ-GT and also total bilurubin; as well as increases the total serum proteins (TP) as compared to animals treated only with CCl4 (group-II). The results of biochemical parameters and histopathological studies suggest that, MEAL possesses significant antidiabetic property and hepatoprection property.

Keywords: Alpinia Leptostachya, Antidiabetic, hepatoprotective, Alloxan and CCl4 etc.